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This bacterial growth medium was developed in 1971 for ''Lactococcus'' species isolated from milk products. It was originally called M16 medium,〔Lowrie, R. J., and L. E. Pearce. (1971). The plating efficiency of bacteriophages of lactic streptococci. N.Z. J. Dairy Sci. Technol. 6:166-171.〕 but in 1975 Terzaghi and Sandine〔Terzaghi B. E., and W. E. Sandine. (1975 ). Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. Jun;29(6):807-13. PubMed PMID 16350018〕 added disodium-β-glycerophosphate to the medium as a buffer, and named the new growth medium M17 medium. It was later found that the addition of disodium-β-glycerophosphate inhibits the growth of many ''Lactobacillus'' species〔Shankar, P. A., and F. L. Davies. (1977). A note on the suppression of Lactobacillus bulgaricus in media containing 0-glycerophosphate and application of the media to selective isolation of Streptococcus thermophilus from yoghurt. J. Soc. Dairy Technol. 30( 1 ): 28.〕 ==Typical composition== Per 950 ml:〔Terzaghi B. E., and W. E. Sandine. (1975 ). Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. Jun;29(6):807-13. PubMed PMID 16350018〕 *5.0 g Pancreatic Digest of Casein *5.0 g Soy Peptone *5.0 g Beef Extract *2.5 g Yeast Extract *0.5 g Ascorbic Acid *0.25 g Magnesium Sulfate *10.0 g Disodium-β-glycerophosphate *11.0 g Agar Preparation: *1. Heat with frequent agitation and boil for 1 minute to completely dissolve. *2. Autoclave at 121°C for 15 minutes. Cool to 50°C. *3. Add 50 ml filter sterilized 10% lactose solution and mix well (the lactose can be exchanged to other carbohydrates e.g. glucose, resulting in GM17 medium) == References == 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「M17 agar」の詳細全文を読む スポンサード リンク
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